Use of an Automatic Methane Potential Test System for evaluating the biomethane potential of sugarcane bagasse after different treatments

► Automatic methane potential test system (AMPTS) was used to evaluate biogas potential of sugarcane bagasse (SCB). ► The reliability of AMPTS was confirmed by the reproducible results obtained during anaerobic digestion of sodium acetate. ► Combined pretreatment with dilute acid and enzymatic hydrolysis resulted in conversion of 79% monomeric sugars from SCB. ► Highest methane yield (+200%) was obtained from acid and enzyme treated SCB, compared to that of raw bagasse. ► The effect of enzymatic saccharification in acid pretreated SCB increased the methane yield by 16%.