Hydrolysis and acidification of grass silage in leaching bed reactors

► Leachate dilution and pH adjustment are the most important factors to LBR performance. ► Inoculum addition is an important but not a critical operation factor. ► Cellulase activity can be used as a key indicator to monitor LBR performance. ► Methane production in LBRs accounted for 10.0-13.8% of BMPs of GS.