Fed-batch fermentation and supercritical fluid extraction of heterotrophic microalgal Chlorella protothecoides lipids

► A glucose fed-batch cultivation in a 7-l fermenter was utilized to increase final biomass and lipid yield. ► The biomass productivity and lipid productivity were 6.28 and 2.06 g/L day, respectively. ► The Goto model was applied for describing the extraction behavior and fitting the experimental overall extraction curve.