Respirometric evaluation and modelling of acetate utilization in sequencing batch reactor under pulse and continuous feeding

► Continuous acetate feeding induces and should be evaluated for substrate storage. ► Lower sludge age increases growth rate for acetate and slightly reduces storage rate. ► Continuous feeding lowers substrate affinity/increases half saturation coefficient. ► Simultaneous growth and storage reduces the maximum growth rate for acetate. ► Variable growth/storage kinetics affects process selection and system optimization.