Lipid production for second generation biodiesel by the oleaginous yeast Rhodotorula graminis

► Rhodotorula graminis can use a broad range of carbon sources for lipid production. ► Yeast biomass with high lipid content can be obtained in fed-batch process. ► High lipid concentration and productivity can be obtained from sugars and glycerol. ► R. graminis can resist to inhibitors by the hydrolysis of lignocellulosic materials. ► R. graminis can be used to produce second generation biodiesel.