Efficient lipase purification using reverse micellar extraction

► Pseudomonas lipase purified using AOT/isooctane reverse micellar system (RME). ► Eighty percentage yield with 14-fold purification and threefold concentration achieved. ► Lipase pI found to regulate forward extraction but not the backward extraction. ► RME is faster than conventional method, scalable and hence industrially significant.