A comparative study of β-1, 4-endoglucanase (possessing β-1, 4-exoglucanase activity) from Bacillus subtilis LH expressed in Pichia pastoris GS115 and Escherichia coli Rosetta (DE3)

► The activity of EGE expressed in Escherichia coli Rosetta (DE3) reached 20,010 U/ml. ► EGP was not degraded by papain and was more thermostable. ► EGE and EGP possessed β-1, 4-endoglucanase and β-1, 4-exoglucanase activities. ► EGE and EGP performed much better than EGT in denim biostoning process.