Biotransformation of methyl-β-d-glucopyranoside to higher chain alkyl glucosides by cell bound β-glucosidase of Pichia etchellsii

► Whole cell biocatalysis using lyophilized cells of Pichia etchellsii without any chemical treatment. ► Biotransformation of Methyl glucoside into Hexyl glucoside, Octyl glucoside, Decyl glucoside and Dodecyl glucoside. ► Optimization of HPLC operating conditions for analysis of higher chain length alkyl glucosides. ► Designing of reactor for large scale synthesis of alkyl glucosides. ► Use of inexpensive glucosyl donor i.e., methyl glucoside for large scale synthesis of higher chain length alkyl glucoside.