Cloning and expression of a gene with phospholipase B activity from Pseudomonas fluorescens in Escherichia coli

► This is the first report on the cloning of a plb gene from the genus Pseudomonas. ► Pf-PLB from P. fluorescens BIT-18 is a member of a new phospholipase B family. ► The overproduction of Pf-PLB is of interest for the applications in many industries.