کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7228792 | 1470885 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Induction and catabolite repression of cellulase and xylanase synthesis in the selected white-rot basidiomycetes
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
مهندسی پزشکی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This paper reports regulation of endoglucanase (EC 3.2.1.4) and xylanase (EC 3.2.1.8) production in submerged cultivation of four white-rot basidiomycetes. Among carbon sources tested, the Avicel-based medium provided the highest levels of both hydrolases activities in all fungal cultures. However, the maximum endoglucanase and xylanase activities of the tested basidiomycetes varied from 3.9Â U/ml and 7.4Â U/ml in Fomes fomentarius to 34.2Â U/ml and 29.5Â U/ml in Pseudotrametes gibbosa, respectively (P. gibbosa specific cellulase and xylanase activities achieved 8.55 and 7.38Â U/mg, respectively). Replacement of Avicel in the medium with carboxymethyl cellulose or xylan significantly lowered the enzyme yield of the tested fungi. Moreover, xylan did not ensure high xylanase activity of these fungi. Lignocellulosic substrates used as a carbon source provided poorer productivity (the specific CMCase activity was 1.12-3.62Â U/mg and the specific xylanase activity was 1.95-3.32Â U/mg). Expression of endoglucanase and xylanase synthesis in Panus lecometei and P. gibbosa was inducible; supplementation of the glycerol-containing medium with Avicel accompanied with a sharp increase of the fungal specific CMCase and xylanase activities from 0.02-0.04Â U/mg to 1.30-8.55Â U/mg. Supplementation of the Avicel-induced cultures with glucose or glycerol caused a catabolite repression of the cellulase and xylanase formation by P. gibbosa and P. lecometei. The enzyme synthesis resumed only after depletion of easily metabolizable carbon source, glucose or glycerol, from the medium. The data received suggest that in the tested fungi endoglucanase and xylanase synthesis is under control by a common regulatory mechanism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Annals of Agrarian Science - Volume 14, Issue 3, September 2016, Pages 169-176
Journal: Annals of Agrarian Science - Volume 14, Issue 3, September 2016, Pages 169-176
نویسندگان
Aza Kobakhidze, Mikheil Asatiani, Eva Kachlishvili, Vladimir Elisashvili,