کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7229036 | 1470915 | 2018 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Aptamer cell sensor based on porous graphene oxide decorated ion-selective-electrode: Double sensing platform for cell and ion
ترجمه فارسی عنوان
سنسور سلول آپتامر بر اساس اکسید گرافن متخلخل تزریقی الکترودهای یونی انتخابی: پلت فرم دو حسگر برای سلول و یون
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کلمات کلیدی
الکترودهای انتخاب یونی، اکسید گرافن متخلخل، آپتامر، سنسور سلولی یدید یون،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Enlightened by the emerging cell-ion detection based on ion-selective-electrode (ISE), an aptamer capturing and ISE transducing (AC&IT) strategy is proposed on the porous graphene oxide (PGO) decorated ISE (PGO-ISE), its performances in both cell and ion detections are examined by use of AS1411 targeted A549 cell detection and iodide-ISE as proof-of-concept. Firstly, GO flakes, exfoliated from graphite by modified Hummers method, are cross-linked by thiourea mediated hydrothermal process, to 3-dimension networked PGO which is identified by scanning-electron-microscope, UV-visible absorbance and X-ray photoelectron spectroscopy; its enhancing effect for cell capturing is evaluated by microscopy. Then, PGO-ISE is constructed by drop-coating PGO film on the surface of ISE and followed by covalently anchoring AS1411. Electrochemistry measurements for different state ISE (blank, PGO coated, AS1411 anchored and A549 captured) are performed by our home-made ISE-measuring system. It is demonstrated that the best cell-sensitivity in buffer is ââ¯25.21â¯mV/log10CA549 (R2 =â¯0.91), resolution in blood is 10 cells/ml. Interestingly, due to PGO's scaffold protection to the ionophore, I--sensitivity is preserved as ââ¯42.98â¯mV/pI (R2 =â¯0.95, pIâ¯=â¯-log10(CI)). Theoretical explanations are provided for the double-sensing phenomenon according to basic ISE principle. It is believed the PGO-ISE based aptamer cell sensor will be a promising experimental means for biomedical researches.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 117, 15 October 2018, Pages 303-311
Journal: Biosensors and Bioelectronics - Volume 117, 15 October 2018, Pages 303-311
نویسندگان
Rong Zhang, Yajun Gu, Zhongrong Wang, Yueguo Li, Qingjie Fan, Yunfang Jia,