کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7229158 | 1470917 | 2018 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dual-wavebands-resolved electrochemiluminescence multiplexing immunoassay with dichroic mirror assistant photomultiplier-tubes as detectors
ترجمه فارسی عنوان
آزمایش ایمونولوژی چندگانه الکتروکیمیلومینسانس حلقوی موج دوگانه با عیب دیجیتال معکوس فوتوولتیمتر-لوله به عنوان آشکارساز
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A dual-wavebands-resolved electrochemiluminescence (ECL) multiplexing immunoassay (MIA) was developed for simultaneously detecting alpha fetoprotein antigen (AFP) in greenish waveband with CdSe550 (λmax = 550â¯nm) nanocrystals (NCs) and carbohydrate antigen 125 (CA125) in near-infrared waveband with CdTe790 (λmax = 790â¯nm) NCs via one-pot ECL reaction, in which dichroic mirror works as a key part to reflect ECL from CdSe550 to one photomultiplier-tube (PMT) and transmit ECL from CdTe790 to the other PMT for dual-wavebands-resolved assay. The proposed ECL-MIA strategy was capable of simultaneously determining AFP with linearly response from 5â¯pg/mL to 5â¯ng/mL and limit of detection at 1â¯pg/mL, and CA125 with linearly response from 5 mU/mL to 1â¯U/mL and limit of detection at 1 mU/mL, with desired specificity and without obvious energy-transfer between ECL tags. The dichroic mirror assistant ECL setup is easy-to-assemble and convenient for the popularization of color-resolved multiplexing ECL assay.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 115, 15 September 2018, Pages 77-82
Journal: Biosensors and Bioelectronics - Volume 115, 15 September 2018, Pages 77-82
نویسندگان
Fang Zhang, Yupeng He, Kena Fu, Li Fu, Bin Zhang, Huaisheng Wang, Guizheng Zou,