کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7229162 | 1470917 | 2018 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sample-to-answer palm-sized nucleic acid testing device towards low-cost malaria mass screening
ترجمه فارسی عنوان
دستگاه تست اسید نوکلئیک به دست آمده از نمونه به پاسخ به مقیاس غربالگری توده کم هزینه مالاریا
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کلمات کلیدی
آزمون اسید نوکلئیک، مالاریا، نقطه مراقبت، خون، سانتریفیوژ رایگان آزمایشگاه روی یک دیسک،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The effectiveness of malaria screening and treatment highly depends on the low-cost access to the highly sensitive and specific malaria test. We report a real-time fluorescence nucleic acid testing device for malaria field detection with automated and scalable sample preparation capability. The device consists a compact analyzer and a disposable microfluidic reagent compact disc. The parasite DNA sample preparation and subsequent real-time LAMP detection were seamlessly integrated on a single microfluidic compact disc, driven by energy efficient non-centrifuge based magnetic field interactions. Each disc contains four parallel testing units which could be configured either as four identical tests or as four species-specific tests. When configured as species-specific tests, it could identify two of the most life-threatening malaria species (P. falciparum and P. vivax). The NAT device is capable of processing four samples simultaneously within 50â¯min turnaround time. It achieves a detection limit of ~0.5 parasites/µl for whole blood, sufficient for detecting asymptomatic parasite carriers. The combination of the sensitivity, specificity, cost, and scalable sample preparation suggests the real-time fluorescence LAMP device could be particularly useful for malaria screening in the field settings.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 115, 15 September 2018, Pages 83-90
Journal: Biosensors and Bioelectronics - Volume 115, 15 September 2018, Pages 83-90
نویسندگان
Gihoon Choi, Theodore Prince, Jun Miao, Liwang Cui, Weihua Guan,