کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7229185 1470918 2018 23 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Patchy gold coated Fe3O4 nanospheres with enhanced catalytic activity applied for paper-based bipolar electrode-electrochemiluminescence aptasensors
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Patchy gold coated Fe3O4 nanospheres with enhanced catalytic activity applied for paper-based bipolar electrode-electrochemiluminescence aptasensors
چکیده انگلیسی
In this work, novel multifunctional patchy gold coated Fe3O4 hybrid nanoparticles (PG-Fe3O4 NPs) have been successfully synthesized in aqueous medium via a facile adsorption-reduction method. A rational formation mechanism has been proposed by monitoring the morphological evolution. The PG-Fe3O4 NPs retained the good magnetic property and exhibited excellent catalytical effeciency towards the electrochemical reduction of hydrogen peroxide. Chronoamperometric and amperometric experiments indicated a relatively high catalytic rate constant of 3.13 × 105 M−1 s−1, a high sensitivity of 578.87 µA mM−1 cm−2 and a low Michaelis-Menten constant of 462 µM. Meanwhile, the introduction of patchy gold could help biofunctionalization via Au-S bond for different biodetection and biosensing purposes. Here, as an example, thiol-terminated aptamers were immobilized onto the patchy gold part as a signal probe to detect carcinoembryonic antigen (CEA). A related paper-based bipolar electrode-electrochemiluminescence (pBPE-ECL) aptasensor was fabricated as the low-cost, disposable and miniature platform. To improve the sensitivity, Au nanodendrites were electrodeposited at the BPE cathode as the matrix for Apt1 immobilization. This aptasensor showed a wide linear range of 0.1 pg mL−1-15 ng mL−1 with a low detection limit of 0.03 pg mL−1, remaining competitive against other ones, and also demonstrating the PG-Fe3O4 NPs have promising potential for catalysis and bioassays.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 114, 30 August 2018, Pages 44-51
نویسندگان
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