کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7229254 1470920 2018 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid, highly sensitive detection of Gram-negative bacteria with lipopolysaccharide based disposable aptasensor
ترجمه فارسی عنوان
تشخیص سریع و حساس باکتری های گرم منفی با آپاتسنسوری یکبار مصرف مبتنی بر لیپوپلی ساکارید
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Gram-negative bacteria are one of the most common microorganisms in the environment. Their differential detection and recognition from Gram-positive bacteria has been attracting much attention over the years. Using Escherichia coli (E. coli) as a model, we demonstrated on-site detection of Gram-negative bacteria by an AC electrokinetics-based capacitive sensing method using commercial microelectrodes functionalized with an aptamer specific to lipopolysaccharides. Dielectrophoresis effect was utilized to enrich viable bacteria to the microelectrodes rapidly, achieving a detection limit of 102 cells/mL within a 30 s′ response time. The sensor showed a negligible response to Staphylococcus aureus (S. aureus), a Gram-positive species. The developed sensor showed significant advantages in sensitivity, selectivity, cost, operation simplicity, and response time. Therefore, this sensing method has shown great application potential for environmental monitoring, food safety, and real-time diagnosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 112, 30 July 2018, Pages 48-53
نویسندگان
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