کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7229679 | 1470926 | 2018 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Label-free surface plasmon resonance biosensing with titanium nitride thin film
ترجمه فارسی عنوان
بیوسنز رزونانس سطح پلاسما سطح بدون برچسب با فیلم نازک تیتانیوم نیترید
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کلمات کلیدی
رزونانس پلاسما سطحی، نیترید تیتانیوم، بیوسنسور، بیوتین، تئوری کاربردی تراکم،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this report, titanium nitride thin film synthesized with reactive magneto-sputtering technique is proposed as an alternative surface plasmon resonance sensing material. The physical and chemical natures were initially studied by atomic force microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. In virtue of white-light common-path sensing system, the wavelength modulated TiN films achieved tunable evanescent plasmonic field from 573â¯nm to 627â¯nm. The optimized TiN film with 29.8â¯nm thickness exhibited good differential phase sensitivity (i.e. 1.932â¯Ãâ¯10â7 RIU) to refractive index alteration, which is comparable to the performance of gold film. We have also attained direct measurement of biotin adsorption on the TiN and monitored sub-sequential biotin-streptavidin conjugation. It was found that TiN films have significantly higher binding affinity toward biotin than that of gold in experiments, so we are able to detect biotin directly to 0.22â¯Âµg/ml (0.90â¯ÂµM) in label-free manner. The adsorption mechanism of biotin on TiN(200) are also explored with periodic density functional theory (DFT) via computer simulation and it was found that the exceptional biotin-TiN affinity may be due to the stacking formation of both N-Ti and O-Ti bonds. Also, the adsorption energy of biotin-TiN was found to be ââ¯1.85â¯eV, which was two times higher than that of biotin-gold. Both experimental and computational results indicate, for the first time, that the TiN film can be directly functionalized with biotin molecules, thus it serves as an alternative plasmonic material to existing gold-based SPR biosensors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 106, 30 May 2018, Pages 129-135
Journal: Biosensors and Bioelectronics - Volume 106, 30 May 2018, Pages 129-135
نویسندگان
Guangyu Qiu, Siu Pang Ng, Chi-Man Lawrence Wu,