کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7229792 1470928 2018 26 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Paper-based fluorescent sensor via aggregation induced emission fluorogen for facile and sensitive visual detection of hydrogen peroxide and glucose
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Paper-based fluorescent sensor via aggregation induced emission fluorogen for facile and sensitive visual detection of hydrogen peroxide and glucose
چکیده انگلیسی
Hydrogen peroxide (H2O2), an important reactive oxygen species (ROS), is related to the oxidative stress in organisms, and plays important roles in a variety of cellular activities as well. So it is of crucial importance to develop sensitive and accurate sensing strategies to detect H2O2 in biological systems. Herein, by taking advantage of the unique emission characteristics of aggregation induced emission (AIE) fluorogens, we proposed a non-enzymatic fluorescence platform for facile and sensitive detection of H2O2, both in solution state using fluorescence spectrometer and on paper-based sensor via visual inspection. Through the reaction between L-cysteine and H2O2, the fluorescence of TPE-M-L, an AIE fluorogen formed between maleimide-functionalized tetraphenylethene (TPE-M) and L-cysteine, is quenched, and highly sensitive non-enzymatic H2O2 assay is readily carried out. The limit of detection (LOD) of 10 nM in solution state and 2.5 μM on paper-based sensor were obtained for H2O2 detection, which were superior or comparable to those previously reported in literature. Moreover, by integrating glucose oxidase with the AIE fluorogen of TPE-M-L, highly sensitive and selective glucose detection was also conveniently achieved both in solution state and on paper-based sensor by the as-proposed strategy, with the LODs of 50 nM in solution state and 10 μM via visual observation, much better than those obtained by other fluorescence methods. The as-proposed sensing strategy was also successfully applied to assay glucose in human serum samples. Therefore, the paper-based fluorescence sensor exhibits the advantages of simple fabrication, high sensitivity and portability, and has great potential to be applied in on-site assay of H2O2 and glucose in real samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 104, 1 May 2018, Pages 152-157
نویسندگان
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