کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230149 | 1470945 | 2016 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel, sensitive and label-free loop-mediated isothermal amplification detection method for nucleic acids using luminophore dyes
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A novel, sensitive and label-free loop-mediated isothermal amplification detection method for nucleic acids using luminophore dyes A novel, sensitive and label-free loop-mediated isothermal amplification detection method for nucleic acids using luminophore dyes](/preview/png/7230149.png)
چکیده انگلیسی
Electrochemiluminescence (ECL) has been widely rendered for nucleic acid testing. Here, we integrate loop-mediated isothermal amplification (LAMP) with ECL technique for DNA detection and quantification. The target LAMP DNA bound electrostatically with [Ru(bpy)3]+2 on the carbon electrode surface, and an ECL reaction was triggered by tripropylamine (TPrA) to yield luminescence. We illustrated this method as a new and highly sensitive strategy for the detection of sequence-specific DNA from different meat species at picogram levels. The proposed strategy renders the signal amplification capacities of TPrA and combines LAMP with inherently high sensitivity of the ECL technique, to facilitate the detection of low quantities of DNA. By leveraging this technique, target DNA of Sus scrofa (pork) meat was detected as low as 1 pg/µL (3.43Ã10â1 copies/µL). In addition, the proposed technique was applied for detection of Bacillus subtilis DNA samples and detection limit of 10 pg/µL (2.2Ã103 copies/µL) was achieved. The advantages of being isothermal, sensitive and robust with ability for multiplex detection of bio-analytes makes this method a facile and appealing sensing modality in hand-held devices to be used at the point-of-care (POC).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 86, 15 December 2016, Pages 346-352
Journal: Biosensors and Bioelectronics - Volume 86, 15 December 2016, Pages 346-352
نویسندگان
Sharmili Roy, Sim Xiao Wei, Jean Liew Zhi Ying, Mohammadali Safavieh, Minhaz Uddin Ahmed,