کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7230383 1470947 2016 23 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Magnetic immunoassay for cancer biomarker detection based on surface-enhanced resonance Raman scattering from coupled plasmonic nanostructures
ترجمه فارسی عنوان
ایمن سازی مغناطیسی برای تشخیص زیست سنجی سرطانی بر اساس رزونانس افزایش سطح پراکندگی رامان از نانوساختارهای پلاسمونی متصل
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A surface-enhanced resonance Raman scattering (SERRS) sensor was developed for the ultrasensitive detection of cancer biomarkers. Capture antibody-coated silver shell magnetic nanoparticles (Fe3O4@Ag MNPs) were utilized as the CEA enrichment platform and the SERRS signal amplification substrate. Gold nanorods (AuNRs) were coated with a thin silver shell to be in resonance with the resonant Raman dye diethylthiatricarbocyanine iodide (DTTC) and the excitation wavelength at 785 nm. The silver-coated AuNRs (Au@Ag NRs) were then modified with detection antibody as the SERRS tags. Sandwich immune complexes formed in the presence of the target biomarker carcinoembryonic antigen (CEA), and this formation induced the plasmonic coupling between the Au@Ag NRs and Fe3O4@Ag MNPs. The SERRS signal of DTTC molecules located in the coupled plasmonic nanostructures was significantly enhanced. As a result, the proposed SERRS sensor was able to detect CEA with a low limit of detection of 4.75 fg/mL and a wide dynamic linear range from 10 fg/mL to 100 ng/mL. The sensor provides a novel SERRS strategy for trace analyte detection and has a potential for clinical applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 84, 15 October 2016, Pages 15-21
نویسندگان
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