کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230420 | 1470946 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lab-on-a-disc agglutination assay for protein detection by optomagnetic readout and optical imaging using nano- and micro-sized magnetic beads
ترجمه فارسی عنوان
تست آگلوتیناسیون آزمایشگاهی بر روی یک دیسک برای تشخیص پروتئین با تشخیص اپتومغناطیس و تصویربرداری نوری با استفاده از دانه های مغناطیسی نانو و میکرو
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
We present a biosensing platform for the detection of proteins based on agglutination of aptamer coated magnetic nano- or microbeads. The assay, from sample to answer, is integrated on an automated, low-cost microfluidic disc platform. This ensures fast and reliable results due to a minimum of manual steps involved. The detection of the target protein was achieved in two ways: (1) optomagnetic readout using magnetic nanobeads (MNBs); (2) optical imaging using magnetic microbeads (MMBs). The optomagnetic readout of agglutination is based on optical measurement of the dynamics of MNB aggregates whereas the imaging method is based on direct visualization and quantification of the average size of MMB aggregates. By enhancing magnetic particle agglutination via application of strong magnetic field pulses, we obtained identical limits of detection of 25 pM with the same sample-to-answer time (15 min 30 s) using the two differently sized beads for the two detection methods. In both cases a sample volume of only 10 µl is required. The demonstrated automation, low sample-to-answer time and portability of both detection instruments as well as integration of the assay on a low-cost disc are important steps for the implementation of these as portable tools in an out-of-lab setting.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 85, 15 November 2016, Pages 351-357
Journal: Biosensors and Bioelectronics - Volume 85, 15 November 2016, Pages 351-357
نویسندگان
Rokon Uddin, Robert Burger, Marco Donolato, Jeppe Fock, Michael Creagh, Mikkel Fougt Hansen, Anja Boisen,