کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230425 | 1470947 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Construction of single fluorophore ratiometric pH sensors using dual-emission Mn2+-doped quantum dots
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We present a novel ratiometric pH sensor design using water-soluble, dual-emission, Mn2+-doped quantum dots (Qdots) decorated with D-penicillamine (DPA-MnQdots). In contrast to more commonly used ratiometric pH sensors that rely on the coupling of two fluorophores, our design uses only a single emitter, which simplifies ratiometric sensing and broadens the applications of the sensor. Our single-emitter DPA-MnQdots exhibit two emission bands, at 510Â nm (green) and 610Â nm (red), which are, respectively, attributable to exciton recombination and emission of the Mn2+ dopants. The emission intensity ratio (I510/I610) of the DPA-MnQdots depends linearly on surrounding pH values within physiological conditions (from pH 4.5 to 8.5). Moreover, the biocompatible DPA-MnQdots were used for long-term monitoring of local pH values in HeLa cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 84, 15 October 2016, Pages 133-140
Journal: Biosensors and Bioelectronics - Volume 84, 15 October 2016, Pages 133-140
نویسندگان
Feby Wijaya Pratiwi, Chih-Hao Hsia, Chiung Wen Kuo, Shun-Min Yang, Yeu-Kuang Hwu, Peilin Chen,