کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230441 | 1470946 | 2016 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sensitive NADH detection in a tumorigenic cell line using a nano-biosensor based on the organic complex formation
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Sensitive NADH detection in a tumorigenic cell line using a nano-biosensor based on the organic complex formation Sensitive NADH detection in a tumorigenic cell line using a nano-biosensor based on the organic complex formation](/preview/png/7230441.png)
چکیده انگلیسی
A robust amperometric sensor for β-nicotinamide adenine dinucleotide (NADH) detection was developed through the organic complex formation with ethylenediaminetetraacetic acid (EDTA) bonded on the polyethylenimine (PEI)/activated graphene oxide (AGO) layer. The EDTA immobilized sensor probe (GCE/AGO/PEI-EDTA) revealed a catalytic property towards NADH oxidation that allows for the highly sensitive electrochemical detection of NADH at a low oxidation potential. Surface characterization demonstrated that the negatively charged AGO acted as nanofillers in the positively charged PEI matrix through the charge interaction. The immobilization of EDTA on the polymer layer provided more surface area for NADH to interact with through the enhanced chemical interlocking between them. We observed the strong interaction between NADH and EDTA on the AGO/PEI layer using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS), and the calculation of the minimized energy for complex formation. The dynamic range of NADH was determined to be between 0.05 μM and 500 μM with a detection limit (LD) of 20.0±1.1 nM. The reliability of the developed sensor for biomedical applications was examined by detecting NADH in tumorigenic lung epithelial cells using the standard addition method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 85, 15 November 2016, Pages 488-495
Journal: Biosensors and Bioelectronics - Volume 85, 15 November 2016, Pages 488-495
نویسندگان
Mahmood H. Akhtar, Tanveer A. Mir, N.G. Gurudatt, Saeromi Chung, Yoon-Bo Shim,