کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230551 | 1470950 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CB[7]-mediated signal amplification approach for sensitive surface plasmon resonance spectroscopy
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cucurbit[7]uril (CB[7]) has received increasing attention because of its unique structure and multiple recognition properties. To improve the sensitivity of surface plasmon resonance (SPR) biosensors, we designed a novel strategy in which caspase-3 serves as the model analyte and CB[7]-modified AuNPs (CB[7]-AuNPs) act as the intermedium. The substrate peptides can be cleaved and replaced with a new N-terminal Phe residue in presence of caspase-3. The CB[7]-AuNPs combine with the N-terminal Phe on the gold chip surface through incorporating the side chain within the nonpolar CB[7] cavity and chelating the N-terminal ammonium group with CB[7] carbonyl oxygen. Then CB[7]-AuNPs integrate with short peptide-modified AuNPs containing Phe at the N-terminal of the peptide. SPR signals are significantly improved through the layer-by-layer assembly of AuNPs. The well-designed sensing platform allows the detection of caspase-3 in a linear range from 10Â fg/mL to 103Â fg/mL with a detection limit of 2.2 fg/mL. Given its high specificity and desirable sensitivity, this CB[7]-assisted SPR method may be a useful tool for the assay of caspase-3 in the future. This work may also afford a new model to improve the sensitivity and selectivity of SPR biosensors in other protein detection experiments and disease diagnosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 81, 15 July 2016, Pages 207-213
Journal: Biosensors and Bioelectronics - Volume 81, 15 July 2016, Pages 207-213
نویسندگان
Yanmin Gao, Fei Zou, Beiping Wu, Xingxin Wang, Jiangjiang Zhang, Kwangnak Koh, Hongxia Chen,