کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230651 | 1470951 | 2016 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Highly sensitive and selective electrochemical detection of Hg2+ through surface-initiated enzymatic polymerization
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A Hg2+ electrochemical biosensor is developed by integrating thymine-Hg2+-thymine (T-Hg2+-T) base pairs for the high selectivity with surface-initiated enzymatic polymerization (SIEP) for signal amplification. The fabrication begins with the covalent conjugation of capture DNA probe labeled with thiol at its 3â²terminal onto the gold electrode. The presence of Hg2+ leads to DNA hybridization, in which complementary DNA was captured onto the biosensor surface, which subsequently catalyzed the addition of deoxynucleotides (dNTP) containing biotinlated 2â²-deoxyadenosine 5â²-triphosphate (biotin-dATP) by terminal deoxynucleotidyl transferase (TdT). The binding between biotin and strepavidin leads to the attachment of a large number of strepavidin functionalized silver nanoparticles (strepavidin-AgNPs), which could generate electrochemical stripping signal of silver to monitor the concentration of Hg2+ in KCl solution. Through utilizing the T-Hg2+-T selectivity and SIEP amplification, this assay method can detect aqueous Hg2+ with a wide linear range from 0.05Â nM to 100Â nM and a detection limit of 0.024Â nM. The application of this sensor in the analysis of drinking water demonstrates that the proposed method works well for real samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 80, 15 June 2016, Pages 105-110
Journal: Biosensors and Bioelectronics - Volume 80, 15 June 2016, Pages 105-110
نویسندگان
Chenyang Mei, Dajie Lin, Chengchao Fan, Aili Liu, Shun Wang, Jichang Wang,