کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230716 | 1470950 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a label-free SPR sensor for detection of matrixmetalloproteinase-9 by antibody immobilization on carboxymethyldextran chip
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Surface plasmon resonance (SPR) immunosensor has been widely utilized for monitoring antigen-antibody interactions. The sensor measures changes of refractive index upon binding of analyte molecules to specific ligand immobilized on the sensor chip. This effort reports development of SPR immunosensor for real-time and label-free detection of recombinant human matrix metalloproteinases-9 (MMP-9), which has been associated with malignant tumor progression and metastasis by matrix degradation. MMP-9 was expressed in Escherichia coli BL21 and purified by Ni-NTA agarose column. CMD 50 D was activated by EDC/NHS for immobilization of monoclonal anti-MMP-9. Atomic force microscopy images showed uniform distribution of anti-MMP-9 over the sensor chip. Equilibrium constant (KD), maximum binding capacity (Rmax) and âGb values for interaction of MMP-9 and anti-MMP-9 were 0.4 nM, 680 µRIU and â53.51 kJ/mol, respectively. Concentration of MMP-9 in saliva samples was determined, with linearity in the range of 10-200 ng/mL. The limit of detection was found to be 8 pg/mL, being lower than most of the previously reported techniques.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 81, 15 July 2016, Pages 510-516
Journal: Biosensors and Bioelectronics - Volume 81, 15 July 2016, Pages 510-516
نویسندگان
Sara Mohseni, Tahereh Tohidi Moghadam, Bahareh Dabirmanesh, Safoura Jabbari, Khosro Khajeh,