کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230759 | 1470950 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid lateral-flow immunoassay for the quantum dot-based detection of puerarin
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
In this study, a rapid (within 10 min) quantitative lateral-flow immunoassay using a quantum dots (QDs)-antibody probe was developed for the analysis of puerarin (PUE) in water and biological samples. The competitive immunoassay was based on anti-PUE monoclonal antibody conjugated with QDs (detection reagent). Secondary antibody was immobilized on one end of a nitrocellulose membrane (control line) and PUE-bovine serum albumin conjugate was immobilized on the other end (test line). In the quantitative experiment, the detection results were scanned using a membrane strip reader and a detection curve (regression equation: y=â0.11 ln(x)+0.979, R2=0.9816) representing the averages of the scanned data was obtained. This curve was linear from 1 to 10 μg/mL. The IC50 value was 75.58 ng/mL and the qualitative detection limit of PUE was 5.8 ng/mL. The recovery of PUE added to phosphate-buffered saline and biological samples was in the range of 97.38-116.56%. To our knowledge, this is the first report of the quantitative detection of a natural product by QDs-based immunochromatography, which represents a powerful tool for rapidly screening PUE in plant materials and other biological samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 81, 15 July 2016, Pages 358-362
Journal: Biosensors and Bioelectronics - Volume 81, 15 July 2016, Pages 358-362
نویسندگان
Huihua Qu, Yue Zhang, Baoping Qu, Hui Kong, Gaofeng Qin, Shuchen Liu, Jinjun Cheng, Qingguo Wang, Yan Zhao,