کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230768 | 1470951 | 2016 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Practical, highly sensitive, and regenerable evanescent-wave biosensor for detection of Hg2+ and Pb2+ in water
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A “turn-on” evanescent-wave fiber biosensor based on functional nucleic acids was developed for on-site detection of Hg2+ and Pb2+ in environmental water samples. A two-step strategy was conducted to take advantage of the rapid binding of T-T mismatches with Hg2+ and the cleavage of a substrate by 8-17 DNAzyme with Pb2+ in solution, as well as sensitive DNA detection on a liquid-solid interface based on evanescent wave. The biosensor demonstrated high sensitivity (with a detection limit of 22 pM for Hg2+ and 20Â nM for Pb2+) and selectivity without any signal amplification, rapid detection (within 13Â min per test), low cost (10-20 Chinese yuan per sample), and multiple-cycle regeneration (at least 18 times). The method was also successfully applied on Hg2+ and Pb2+ detection in real environmental water samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 80, 15 June 2016, Pages 265-272
Journal: Biosensors and Bioelectronics - Volume 80, 15 June 2016, Pages 265-272
نویسندگان
Shitong Han, Xiaohong Zhou, Yunfei Tang, Miao He, Xinyu Zhang, Hanchang Shi, Yu Xiang,