کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7230776 1470951 2016 27 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Silver nanoflower-reduced graphene oxide composite based micro-disk electrode for insulin detection in serum
ترجمه فارسی عنوان
الکترودهای میکرو دیسک مبتنی بر کامپوزیت گرافن نقره ای برای تشخیص انسولین در سرم
کلمات کلیدی
نانوفیلور نقره، کاهش اکسید گرافین، انسولین، امپدانس، میکرو دیسک، اکسید قلع ایندیوم،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Sensitive and selective determination of protein biomarkers remains a significant challenge due to the existence of various biomarkers in human body at a low concentration level. Therefore, new technologies were incessantly steered to detect tiny biomarkers at a low concentration level, yet, it is difficult to develop reliable, stable and sensitive detection methods for disease diagnostics. Therefore, the present study demonstrates a methodology to detect insulin in serum at low levels based on Ag nanoflower (AgNF) decorated reduced graphene oxide (rGO) modified micro-disk electrode arrays (MDEAs). The morphology of AgNF-rGO composite was characterized by scanning electron microscopy, the structure was analyzed using X-ray diffraction patterns and Raman spectra. The hybrid interface exhibited enhanced electrical conductivity when compared with its individual elements and had improved capturing ability for antibody-antigen binding towards insulin detection. In order to measure quantitatively the insulin concentration in PBS and human serum, the change in impedance (ΔZ) from electrochemical impedance spectroscopy was analyzed for various concentrations of insulin in [Fe(CN)6]3−/4− redox couple. The electrode with adsorbed antibodies showed an increase in ΔZ for the addition of antigen concentrations over a working range of 1-1000 ng mL−1. The detection limits were 50 and 70 pg mL−1 in PBS and human serum, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 80, 15 June 2016, Pages 307-314
نویسندگان
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