کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230821 | 1470953 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Engineered “hot” core-shell nanostructures for patterned detection of chloramphenicol
ترجمه فارسی عنوان
مهندسی شده؟ نانوساختارهای هسته-پوسته برای تشخیص الگو از کلرامفنیکول
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this study, we described a novel method for highly sensitive and specific detection of chloramphenicol (CAP) based on engineered “hot” Au core-Ag shell nanostructures (Au@Ag NSs). Cy5-labeled DNA aptamer was embedded between the Au and Ag layers as a signal generator and target-recognition element, to fabricate uniform Au@Ag NSs with unexpected strong and stable SERS signals. The presented CAP can specifically bind to the DNA aptamer by forming an aptamer-CAP conjugate, and cause greatly decreased SERS signals of Au@Ag NSs. By using this method, we were able to detect as low as 0.19 pg mLâ1 of CAP with high selectivity, which is much lower than those previously reported biosensors. Compared with the other SERS sensors that attached a dye in the outer layer of nanoparticles, this method exhibits excellent sensitivity and has the potential to significantly improve stability and reproducibility of SERS-based detection techniques.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 78, 15 April 2016, Pages 67-72
Journal: Biosensors and Bioelectronics - Volume 78, 15 April 2016, Pages 67-72
نویسندگان
Wenjing Yan, Longping Yang, Hong Zhuang, Haizhou Wu, Jianhao Zhang,