کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7230821 1470953 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Engineered “hot” core-shell nanostructures for patterned detection of chloramphenicol
ترجمه فارسی عنوان
مهندسی شده؟ نانوساختارهای هسته-پوسته برای تشخیص الگو از کلرامفنیکول
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this study, we described a novel method for highly sensitive and specific detection of chloramphenicol (CAP) based on engineered “hot” Au core-Ag shell nanostructures (Au@Ag NSs). Cy5-labeled DNA aptamer was embedded between the Au and Ag layers as a signal generator and target-recognition element, to fabricate uniform Au@Ag NSs with unexpected strong and stable SERS signals. The presented CAP can specifically bind to the DNA aptamer by forming an aptamer-CAP conjugate, and cause greatly decreased SERS signals of Au@Ag NSs. By using this method, we were able to detect as low as 0.19 pg mL−1 of CAP with high selectivity, which is much lower than those previously reported biosensors. Compared with the other SERS sensors that attached a dye in the outer layer of nanoparticles, this method exhibits excellent sensitivity and has the potential to significantly improve stability and reproducibility of SERS-based detection techniques.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 78, 15 April 2016, Pages 67-72
نویسندگان
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