کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230865 | 1470952 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dual-primer self-generation SERS signal amplification assay for PDGF-BB using label-free aptamer
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Dual-primer self-generation SERS signal amplification assay for PDGF-BB using label-free aptamer Dual-primer self-generation SERS signal amplification assay for PDGF-BB using label-free aptamer](/preview/png/7230865.png)
چکیده انگلیسی
Highly sensitive detection of proteins, especially those associated with cancers, is essential to biomedical research as well as clinical diagnosis. In this work, a simple and novel one-two-three signal amplification surface-enhanced Raman scattering (SERS) method for the detection of protein is fabricated by using label-free aptamer and dual-primer self-generation. Platelet-derived growth factor B-chain (PDGF-BB) is selected as the model protein. The one-two-three cascade DNA amplification means one target-aptamer binding event, two hairpin DNA switches and three DNA amplification reactions. This strategy possesses some remarkable features compared to conventional signal amplification methods: (i) A smart probe including a label-free aptamer is fabricated, for suitable hybridization without hindering the affinity of the aptamer toward its target. (ii) Using the unique structure switch of the aptamer and cooperator, a one-two-three working mode is developed to amplify the SERS signal. The amplification efficiency is enhanced. Given the unique and attractive characteristics, a simple and universal strategy is designed to accomplish ultrasensitive detection of proteins. The detection limit of PDGF-BB via SERS detection is 0.42Â pM, with the linear range from 1.0Ã10â12Â M to 10â8Â M. It is potentially universal because the aptamer can be easily designed for biomolecules whose aptamers undergo similar conformational changes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 79, 15 May 2016, Pages 130-135
Journal: Biosensors and Bioelectronics - Volume 79, 15 May 2016, Pages 130-135
نویسندگان
SuJuan Ye, XiaoMo Zhai, YanYing Wu, ShaoPing Kuang,