کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230979 | 1470952 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Designing anthraquinone-pyrrole redox intercalating probes for electrochemical gene detection
ترجمه فارسی عنوان
طراحی پروبهای تقویت کننده سرطان آندوراکینون-پیررول برای تشخیص ژن الکتروشیمیایی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The real-time quantitative electrochemical monitoring of nucleic acid amplification through PCR is a promising renowned methodology to detect pathogenic DNAs. In this work, anthraquinone-pyrrole derivatives based redox intercalating probes (AP probes: AP1, AP2) have been designed, synthesized, characterized and successfully demonstrated in real-time like quantitative PCR. The rationally designed AP probes exhibited excellent DNA binding abilities and electrochemical behaviors. The binding parameters such as binding constant, binding site size and diffusion coefficient were estimated which were comparable to literature reports. Besides, the AP probes are highly stable under PCR thermal conditions and did not inhibit PCR. Therefore, a real-time like quantification of DNA amplification was demonstrated to quantify the initial copy number of target genes. The probe AP2 has excellent ability to detect ~103 copies of target tpc DNA with good sensitivity. The AP probes are metal-free, easily synthesizable, non-toxic, thermally stable and feasible for miniaturized PCR chips.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 79, 15 May 2016, Pages 294-299
Journal: Biosensors and Bioelectronics - Volume 79, 15 May 2016, Pages 294-299
نویسندگان
Yu-Jen Lin, Yung-Chao Wu, Veerappan Mani, Sheng-Tung Huang, Chih-Hung Huang, Yi-Chiuen Hu, His-Chi Peter Shan,