کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231027 | 1470952 | 2016 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Label-free detection of liver cancer cells by aptamer-based microcantilever biosensor
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Label-free detection of liver cancer cells by aptamer-based microcantilever biosensor Label-free detection of liver cancer cells by aptamer-based microcantilever biosensor](/preview/png/7231027.png)
چکیده انگلیسی
Liver cancer is one of the most common and highly malignant cancers in the world. There are no effective therapeutic options if an early liver cancer diagnosis is not achieved. In this work, detection of HepG2 cells by label-free microcantilever array aptasensor was developed. The sensing microcantilevers were functionalized by HepG2 cells-specific aptamers. Meanwhile, to eliminate the interferences induced by the environment, the reference microcantilevers were modified with 6-mercapto-1-hexanol self-assembled monolayers. The aptasensor exhibits high specificity over not only human liver normal cells, but also other cancer cells of breast, bladder, and cervix tumors. The linear relation ranges from 1Ã103 to 1Ã105Â cells/mL, with a detection limit of 300Â cells/mL (S/N=3). Our work provides a simple method for detection of liver cancer cells with advantages in terms of simplicity and stability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 79, 15 May 2016, Pages 353-358
Journal: Biosensors and Bioelectronics - Volume 79, 15 May 2016, Pages 353-358
نویسندگان
Xuejuan Chen, Yangang Pan, Huiqing Liu, Xiaojing Bai, Nan Wang, Bailin Zhang,