کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231154 | 1470953 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons](/preview/png/7231154.png)
چکیده انگلیسی
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous homogeneous chemicals which are well known by carcinogens, mutagens and endocrine disorder. Here, an improved real-time immuno-PCR (RT-IPCR) was developed for detection of pyrene and its homologs in water samples. The PAHs in sample compete with pyrene-modified DNA to bind with monoclonal antibody (McAb) coated on PCR plate. The reporter DNA was exponentially amplified by real-time PCR instrument using Fast Start universal SYBR Green Master (ROX) kit. Only two reaction steps were needed to accomplish the detection. The assay had a good linear range from 5 pmol Lâ1 to 5 nmol Lâ1 with a detection limit of 3.5 pmol Lâ1. For application assay, the average recoveries from tap water, lake water and mineral water were 98.4%, 98.2% and 99.7%, respectively which showed a good correlation (R2=0.9906) with those from GC-MS. The results indicated that the improved RT-IPCR seems to be a potential method for simple and ultrasensitive detection of pyrene and some homologues in environment water samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 78, 15 April 2016, Pages 194-199
Journal: Biosensors and Bioelectronics - Volume 78, 15 April 2016, Pages 194-199
نویسندگان
X.Y. Meng, Y.S. Li, Y. Zhou, Y. Sun, B. Qiao, C.C. Si, P. Hu, S.Y. Lu, H.L. Ren, Z.S. Liu, H.J. Qiu, J.Q. Liu,