Novel optical sensing film based on a functional nonwoven nanofibre mat for an easy, fast and highly selective and sensitive detection of tryptamine in beer

The proposed sensor is based on a novel white, uncharged, and non-luminescent functional nonwoven nanofibre mat (Tiss®-Link) formed by hydrophilic nanofibres of 300 nm of diameter functionalized with a high concentration of active vinyl groups (330 µmol g−1). It is used to carry out a kinetically controlled covalent immobilisation of tryptamine via Michael type-reaction. The transduction of the sensor is phosphorescence; the covalently immobilized tryptamine is quantified by SS-RTP, obtaining a detection limit of 6 ng mL−1 with short response times (15 min). The applicability of the sensor was demonstrated by analysing tryptamine in 10 different varieties of beers, obtaining recovery percentages close to 100%.