کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231584 | 1470958 | 2015 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Timing readout in paper device for quantitative point-of-use hemin/G-quadruplex DNAzyme-based bioassays
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
This work describes a quantitative point-of-use hemin/G-quadruplex DNAzyme-based assay that integrates a simple timing detection motif with low-cost, portable microfluidic paper-based analytical devices (μPADs). The timing readout is based on the selective DNAzyme-mediated wettability change of paper from hydrophilicity to hydrophobicity. Its utility is well demonstrated with sensitive, specific detection of K+ ion as a model analyte in artificial samples as well as real human serum samples. This new method only requires a ubiquitous cheap timer (or a cell phone with a timing function) to provide quantitative results. It could offer new opportunities for the development of more peroxidase-mimicking DNAzyme-based bioassays that are simple, affordable, portable, and operable by minimally-trained users for broad point-of-use applications especially in resource-poor settings.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 73, 15 November 2015, Pages 13-18
Journal: Biosensors and Bioelectronics - Volume 73, 15 November 2015, Pages 13-18
نویسندگان
Yun Zhang, Jinlong Fan, Jinfang Nie, Shangwang Le, Wenyuan Zhu, Dong Gao, Jiani Yang, Songbai Zhang, Jianping Li,