کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231727 | 1470957 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A label-free fluorescent molecular beacon based on DNA-Ag nanoclusters for the construction of versatile Biosensors
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A label-free fluorescent molecular beacon based on DNA-Ag nanoclusters for the construction of versatile Biosensors A label-free fluorescent molecular beacon based on DNA-Ag nanoclusters for the construction of versatile Biosensors](/preview/png/7231727.png)
چکیده انگلیسی
In this paper, we developed a simple, low-cost and sensitive DNA sequences detection biosensor based on a label-free molecular beacon (MB) whose DNA hairpin structure terminal has a guanine-rich sequence that can enhance fluorescence of silver nanoclusters (Ag NCs). Without hybridization between hairpin probe and target DNA, the Ag NCs presented bright fluorescence for the proximity of guanine-rich sequences (GRSs). After binding with target DNA, the hairpin shape was destroyed which results in a decrease of the Ag NCs fluorescence intensity. With this biosensor, we detected three disease-related genes that were the human immunodeficiency virus (HIV) gene, hepatitis B virus (HBV) gene and human T-lymphotropic virus type I (HTLV-I) gene. The detection limits based on S/N of 3 were 4.4Â nM, 6.8Â nM and 8.5Â nM for HIV gene, HBV gene and HTLV-I gene, respectively. Our sensor was also of high selectivity and could distinguish even one nucleotide mismatched target.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 74, 15 December 2015, Pages 318-321
Journal: Biosensors and Bioelectronics - Volume 74, 15 December 2015, Pages 318-321
نویسندگان
Qiao Cao, Ye Teng, Xuan Yang, Jin Wang, Erkang Wang,