کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231810 | 1470957 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Combination of dynamic magnetophoretic separation and stationary magnetic trap for highly sensitive and selective detection of Salmonella typhimurium in complex matrix
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Foodborne illnesses have always been a serious problem that threats public health, so it is necessary to develop a method that can detect the pathogens rapidly and sensitively. In this study, we designed a magnetic controlled microfluidic device which integrated the dynamic magnetophoretic separation and stationary magnetic trap together for sensitive and selective detection of Salmonella typhimurium (S. typhimurium). Coupled with immunomagnetic nanospheres (IMNs), this device could separate and enrich the target pathogens and realize the sensitive detection of target pathogens on chip. Based on the principle of sandwich immunoassays, the trapped target pathogens identified by streptavidin modified QDs (SA-QDs) were detected under an inverted fluorescence microscopy. A linear range was exhibited at the concentration from 1.0Ã104 to 1.0Ã106Â colony-forming units/mL (CFU/mL), the limit of detection (LOD) was as low as 5.4Ã103Â CFU/mL in milk (considering the sample volume, the absolute detection limit corresponded to 540Â CFU). Compared with the device with stationary magnetic trap alone, the integrated device enhanced anti-interference ability and increased detection sensitivity through dynamic magnetophoretic separation, and made the detection in complex samples more accurate. In addition, it had excellent specificity and good reproducibility. The developed system provides a rapid, sensitive and accurate approach to detect pathogens in practice samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 74, 15 December 2015, Pages 628-636
Journal: Biosensors and Bioelectronics - Volume 74, 15 December 2015, Pages 628-636
نویسندگان
Pei-Lin Guo, Man Tang, Shao-Li Hong, Xu Yu, Dai-Wen Pang, Zhi-Ling Zhang,