کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231994 | 1470961 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel ECL biosensor for β-lactamase detection: Using RU(II) linked-ampicillin complex as the recognition element
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A novel ECL biosensor for β-lactamase detection: Using RU(II) linked-ampicillin complex as the recognition element A novel ECL biosensor for β-lactamase detection: Using RU(II) linked-ampicillin complex as the recognition element](/preview/png/7231994.png)
چکیده انگلیسی
In this work, Ru(phen)2(cpaphen)2+ linked-ampicillin (Ru-Amp), as the novel specific recognition element, was proposed to construct a simple and sensitive electrogenerated chemiluminescence (ECL) biosensor for the determination of β-lactamase. Here, Ru-Amp complex acted not only as a novel specific recognition element for β-lactamase but also as the ECL Luminescent reagent. Through electrostatic adsorption and the intermolecular Ï-Ï interactions, a large amount of Ru-Amp was immobilized to gold nanoparticles (TA@AuNPs) prepared by thiophenemalonic acid (TA) to obtain Ru-Amp/TA@AuNPs nanocomposites. The nanocomposites, which can produce very stable films exhibiting excellent ECL behaviors, were self-assembled on the CNTs-Nf modified glassy carbon electrode surface. The presence of the target β-lactamase resulted in autonomous hydrolysis reaction of Amp, achievement of the efficient ECL emission and highly sensitive detection of β-lactamase. The biosensor for β-lactamase detection was developed with excellent sensitivity of a concentration variation from 50 pg mLâ1 to 100 ng mLâ1 with a low detection limit of 37 pg mLâ1. An ECL assay offers the proposed method opportunities for designing new Ru-based ECL luminophores for biosensing applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 70, 15 August 2015, Pages 221-225
Journal: Biosensors and Bioelectronics - Volume 70, 15 August 2015, Pages 221-225
نویسندگان
Guo-Feng Gui, Ying Zhuo, Ya-Qin Chai, Yun Xiang, Ruo Yuan,