کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7232079 | 1470961 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe](/preview/png/7232079.png)
چکیده انگلیسی
We have synthesized a series of luminescent iridium(III) complexes and investigated their ability to act as luminescent split G-quadruplex probes. After screening, the iridium(III) complex 1 [Ir(2-phenylquinoline)2(3,4,7,8-tetramethyl-1,10-phenanthroline)]PF6 was validated as a highly-selective G-quadruplex probe and was utilized to construct a label-free intermolecular G-quadruplex-based assay for the selective and sensitive detection of LMP1 gene deletion. This “mix-and-detect” assay is simple and selective, and could detect down to 10Â nM of the target gene in aqueous solution with a linear range from 10 to 500Â nM. We also investigated the performance of our split G-quadruplex-based sensing platform for LMP1 gene deletion in the presence of cellular debris, demonstrating the robustness of this sensing system in biological samples. Comparative assays were also performed using either organic dyes or labeled oligonucleotides as signal-transducing agents.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 70, 15 August 2015, Pages 338-344
Journal: Biosensors and Bioelectronics - Volume 70, 15 August 2015, Pages 338-344
نویسندگان
Modi Wang, Bingyong He, Lihua Lu, Chung-Hang Leung, Jean-Louis Mergny, Dik-Lung Ma,