کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7232084 1470960 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sensitive detection for coralyne and mercury ions based on homo-A/T DNA by exonuclease signal amplification
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Sensitive detection for coralyne and mercury ions based on homo-A/T DNA by exonuclease signal amplification
چکیده انگلیسی
Based on specific homo-A/T DNA binding properties, a strategy for coralyne and mercury ions detection was realised by exonuclease-aided signal amplification. Coralyne could specifically bind homo-A DNA and protect it from the hydrolysis of exonuclease I. The coralyne-protected DNA was subsequently used as a trigger strand to hydrolyze DNA2 in exonuclease-aided signal amplification process. Thiazole orange was used to quantify the remainder DNA2. Under the optimal condition, the fluorescence intensity was linearly proportional to the concentration of coralyne in the range of 0.2-100 nM with a limit of detection (LOD) of 0.31 nM, which presented the lowest LOD for coralyne among all reported. With homo-T and Hg2+ taking the place of homo-A DNA and coralyne, respectively, the system could also be used for Hg2+ detection. The experiments in real samples also showed good results. This method was label-free, low-cost, easy-operating and highly repeatable for the detection of coralyne and mercury ions. It could also be extended to detect various analytes, such as other metal ions, proteins and small molecules by using appropriate aptamers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 71, 15 September 2015, Pages 439-444
نویسندگان
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