کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7232252 1644974 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Visible assay for glycosylase based on intrinsic catalytic ability of graphene/gold nanoparticles hybrids
ترجمه فارسی عنوان
تست قابل مشاهده برای گلیکوزیلاز بر اساس توانایی کاتالیزوری ذاتی از هیبرید نانوذرات گرافن / طلا
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A sensitive, rapid and label-free assay for colorimetric detection of human 8-hydroxyguanine glycosylase (hOGG1) was proposed based on the tunable catalytic ability of graphene/gold nanoparticles (graphene/Au-NPs) hybrids and the terminal protection of hOGG1. In presence of H2O2, the hybrids were capable of catalyzing the oxidation of color developing reagent, causing a concomitant change in color. Due to the excellent controllability, the capacity could be inhibited by adsorption of ssDNA onto the hybrids sheets and recovered when the adsorbed ssDNA was digested by exonuclease. The terminal protection of hOGG1 could irreversibly interrupt the digestion of the captured ssDNA (containing the oxidative damage site) by the exonuclease, thus preventing the catalytic ability of graphene/Au-NPs from being recovered. The original color change which related to the concentration of the protected ssDNA facilitated quantitative detection of hOGGl activity. Compared with conventional methods for hOGG1 detection, the presented assay without any labeling process greatly simplified the operation steps and reduced the analysis time. This approach performed a linear response for hOGG1 activity from 0.02 to 0.11 U/μL with a detection limit of 0.0016 U/μL, and realized the quantification of hOGG1 activity in real cell lines.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 68, 15 June 2015, Pages 7-13
نویسندگان
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