کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7232398 | 1470964 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nanochannel array device operating through Prussian blue nanoparticles for sensitive label-free immunodetection of a cancer biomarker
ترجمه فارسی عنوان
دستگاه آرایه نانوکانال که از طریق نانوذرات آبی پروسی به کار می رود برای شناسایی ایمنی حساس بی حس کننده سرطانی
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کلمات کلیدی
نانوذرات آبی پروسی، نانوکانال، بیوسنسور، بیومارکر سرطان،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A novel nanochannel array (NC) device that operates through Prussian blue nanoparticles (PBNPs) as redox indicator for sensitive label free immunodetection of a cancer biomarker is presented. Stable and narrow-sized (around 4 nm) PBNPs, protected by polyvinylpyrrolidone, exhibited a well-defined and reproducible redox behavior and were successfully applied for the voltammetric evaluation of the nanochannels (20 nm pore sized) blockage due to the immunocomplex formation. The bigger size of the PBNPs compared with ionic indicators such as the [Fe(CN)6]4â/3â system leads to an increase in the steric effects hindering their diffusion toward the signaling electrode which in turn is transduced to an improvement of the detection limit from 200 µg mLâ1 to 34 pg human IgG mLâ1. This novel and effective PBNPs-NC technology for the detection of small proteins captured inside the nanochannels is successfully applied for the quantification of a cancer biomarker (parathyroid hormone-related protein, PTHrP) in a real clinical scenario such as cell culture medium. The achieved label-free detection of PTHrP at levels of 50 ng mLâ1 is with great interest to study relevant functions that this protein exerts in normal tissues and cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 67, 15 May 2015, Pages 107-114
Journal: Biosensors and Bioelectronics - Volume 67, 15 May 2015, Pages 107-114
نویسندگان
Marisol Espinoza-Castañeda, Alfredo de la Escosura-Muñiz, Alejandro Chamorro, Carmen de Torres, Arben Merkoçi,