کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7232704 | 1470964 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A smart DNA sensing system for detecting methicillin-resistant Staphylococcus aureus using modified nanoparticle probes
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A smart DNA sensing system for detecting methicillin-resistant Staphylococcus aureus using modified nanoparticle probes A smart DNA sensing system for detecting methicillin-resistant Staphylococcus aureus using modified nanoparticle probes](/preview/png/7232704.png)
چکیده انگلیسی
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common causes of hospital-acquired infections. To prevent epidemics, a quick and simple detection method is required. In this study, we developed a novel electrochemical DNA detection method that does not rely upon polymerase chain reaction (PCR) and may be used in point-of-care facilities. The electrochemical DNA sensing system presented here is based on the chronoamperometric detection of ferrocene-labeled probes that were conjugated to gold nanoparticles (AuNPs). This DNA sensor system employed magnetic nanoparticle (MNP)-modified probes allowing easy sample DNA recovery. AuNP nanoparticles with ferrocene-labeled probes enabled the generation of an electric signal, and MNP/DNA/AuNP conjugates were formed by hybridization. Following hybridization, the MNP/DNA/AuNP hybridization complex is magnetically separated, and electrochemical current responses could be obtained because of the AuNP-ferrocene complexes. To construct a highly sensitive system, dye-linked l-proline dehydrogenase (l-proDH) was employed to amplify current responses following a catalytic reaction with l-proline. Rapid catalytic reaction of l-proDH and substrate was able to amplify the oxidation of ferrocene. Target DNA from MRSA could be quantified over a range of 10-166Â pM, and this sensing system could also distinguish MRSA from S. aureus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 67, 15 May 2015, Pages 419-423
Journal: Biosensors and Bioelectronics - Volume 67, 15 May 2015, Pages 419-423
نویسندگان
Kazuya Watanabe, Noriaki Kuwata, Hiroaki Sakamoto, Yoshihisa Amano, Takenori Satomura, Shin-ichiro Suye,