کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7232726 | 1644974 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Label-free colorimetric detection of biothiols utilizing SAM and unmodified Au nanoparticles
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Label-free colorimetric detection of biothiols utilizing SAM and unmodified Au nanoparticles Label-free colorimetric detection of biothiols utilizing SAM and unmodified Au nanoparticles](/preview/png/7232726.png)
چکیده انگلیسی
Herein, a sensitive and selective sensor for biothiols based on colorimetric assay is reported. S-adenosyl-l-methionine (SAM) could induce the selective aggregation of unmodified gold nanoparticles (AuNPs) by electrostatic interaction. In the presence of biothiols, such as glutathione (GSH), homocysteine (Hcy), and cysteine (Cys), AuNPs prefer to react with thiols of biothiols rather than SAM due to the formation of Au-S bond. Thus, the AuNPs turn from the aggregation to the dispersion state, and the corresponding color variation in the process of anti-aggregation of AuNPs can be used for the quantitative screening of biothiols through UV-vis spectroscopy or by the naked eye. Under optimized conditions, a good linear relationship in the range of 0.4-1.2 µM is obtained for Cys, 0.2-0.9 µM for GSH, and 0.6-3.0 µM for Hcys. The detection limits of this assay for GSH, Cys and Hcys are 35.8 nM, 21.7 nM, and 62.4 nM, respectively. This colorimetric assay exhibits rapid operation (within 5 min), high selectivity and sensitivity towards biothiols with tunable dynamic ranges.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 68, 15 June 2015, Pages 668-674
Journal: Biosensors and Bioelectronics - Volume 68, 15 June 2015, Pages 668-674
نویسندگان
Zhi-Jian Li, Xiang-Juan Zheng, Li Zhang, Ru-Ping Liang, Zhi-Mei Li, Jian-Ding Qiu,