کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7232798 1470965 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultrasensitive immunochromatographic assay for the simultaneous detection of five chemicals in drinking water
ترجمه فارسی عنوان
آزمایش ایمونوچروماتوگرافی حساس به نور برای تشخیص همزمان پنج ماده شیمیایی در آب آشامیدنی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this paper, we describe the development of a multicomponent lateral-flow assay based on an antibody-antigen reaction for the rapid and simultaneous detection of trace contaminants in water, including a heavy metal, algal toxin, antibiotic, hormone, and pesticide. The representative analytes chosen for the study were lead (Pb(II), microcystin-leucine-arginine (MC-LR), chloramphenicol (CAP), testosterone (T), and chlorothalonil (CTN). Five different antigens were immobilized separately in five test lines on a nitrocellulose membrane. The monoclonal antibodies specifically recognized the corresponding antigens, and there was no cross-reactivity between the antibodies in the detection assay. Samples or standards containing the five analytes were preincubated with the freeze-dried colloidal-gold-labeled monoclonal antibody conjugates to improve the sensitivity of the assay. The results were obtained within 20 min with a paper-based sensor. The cut-off values for the strip test were 4 ng/mL for Pb(II), 1 ng/mL for MC-LR, 0.1 ng/mL for CAP, 5 ng/mL for T, and 5 ng/mL for CTN. The assay was evaluated using spiked water samples, and the accuracy and reproducibility of the results were good. In summary, this lateral-flow device provides an effective and rapid method for the onsite detection of multiple contaminants in water samples, with no treatment or devices required.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 66, 15 April 2015, Pages 445-453
نویسندگان
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