کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7233011 1470967 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fabrication of electrochemical interface based on boronic acid-modified pyrroloquinoline quinine/reduced graphene oxide composites for voltammetric determination of glycated hemoglobin
ترجمه فارسی عنوان
ساخت اینترفورد الکتروشیمیایی بر پایه ترکیبات پراکرولووینینین کینین / کاهش اسید گرافن بورون اسید و کاهش ترکیبات اکسید گرافین برای تعیین ولتاژ و تعیین هموگلوبین گلیکوزی
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A voltammetric sensor for determination of glycated hemoglobin (HbA1c) was developed based on the composites of phenylboronic acid-modified pyrroloquinoline quinine (PBA-PQQ) and reduced graphene oxide. After the electrodeposition of reduced graphene oxide (ERGO) on the glassy carbon (GC) electrode, PQQ multilayer was decorated on the surface of the ERGO/GC electrode via potential cycling. Further modification with PBA would lead to the formation of the working electrode, namely PBA-PQQ/ERGO/GC electrode. PQQ on the electrode exhibited a quasi-reversible electrode process with 2-electron transfer and 2-proton participation, and the electron transfer efficiency was further enhanced by the introduction of ERGO layer. The complexation of PBA with HbA1c through specific boronic acid-diol recognition could cause the change of the oxidation peak current of PQQ on the electrode, which was utilized for HbA1c detection. Under the optimized conditions, the PBA-PQQ/ERGO/GC electrode provided high selectivity and high sensitivity for HbA1c detection with a linear range of 9.4-65.8 μg mL−1 and a low detection limit of 1.25 μg mL−1. The fabricated sensor was also successfully applied to determine the percentages of HbA1c in whole blood of healthy individuals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 64, 15 February 2015, Pages 442-448
نویسندگان
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