کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233084 | 1470967 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor for β-agonist residues
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10 min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10 ng/mL, 0.10 ng/mL and 0.09 ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC-MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 64, 15 February 2015, Pages 511-516
Journal: Biosensors and Bioelectronics - Volume 64, 15 February 2015, Pages 511-516
نویسندگان
Peilong Wang, Zhi Wang, Xiaoou Su,