کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7233237 1470969 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Impedance-derived electrochemical capacitance spectroscopy for the evaluation of lectin-glycoprotein binding affinity
ترجمه فارسی عنوان
طیف سنجی ظرفیت الکتروشیمیایی مشتق از امپدانس برای ارزیابی وابستگی لکتین-گلیکوپروتئین
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Characterization of lectin-carbohydrate binding using label-free methods such as impedance-derived electrochemical capacitance spectroscopy (ECS) is desirable to evaluate specific interactions, for example, ArtinM lectin and horseradish peroxidase (HRP) glycoprotein, used here as a model for protein-carbohydrate binding affinity. An electroactive molecular film comprising alkyl ferrocene as a redox probe and ArtinM as a carbohydrate receptive center to target HRP was successfully used to determine the binding affinity between ArtinM and HRP. The redox capacitance, a transducer signal associated with the alkyl ferrocene centers, was obtained by ECS and used in the Langmuir adsorption model to obtain the affinity constant (1.6±0.6)×108 L mol−1. The results shown herein suggest the feasibility of ECS application for lectin glycoarray characterization.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 62, 15 December 2014, Pages 102-105
نویسندگان
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