کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233251 | 1470969 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Target triggered self-assembly of Au nanoparticles for amplified detection of Bacillus thuringiensis transgenic sequence using SERS
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Target triggered self-assembly of Au nanoparticles for amplified detection of Bacillus thuringiensis transgenic sequence using SERS Target triggered self-assembly of Au nanoparticles for amplified detection of Bacillus thuringiensis transgenic sequence using SERS](/preview/png/7233251.png)
چکیده انگلیسی
The research methods for DNA detection have been widely extended since the application of nanotechnology, but it remains a challenge to detect specific DNA sequences or low abundance genes in the biological samples with accuracy and sensitivity. Here we developed a SERS biosensing platform by target DNA (tDNA) triggered self-assembly of Au nanoparticles (Au NPs) probes on DNA nanowires for signal amplification in DNA analysis. Based on the hybridization chain reactions (HCR) and surface enhanced Raman scattering (SERS) technology, the SERS intensity reveals a good linearity with tDNA ranging from 50Â pM to 500Â pM under optimal conditions. The specific detection of tDNA sequence was realized with a detection limit of 50Â pM (S/N=3). To demonstrate the specificity and universality of the strategy, the single-base mismatches in DNA and the Bacillus thuringiensis (Bt) transgenic sequence were successively applied in the SERS assay. The results showed that the sensitivity and accuracy of the SERS-based assay were comparable with real-time PCR. Besides, the method would provide precise and ultra-sensitive detection of tDNA but also informative supplement to the SERS biosensing platform.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 62, 15 December 2014, Pages 196-200
Journal: Biosensors and Bioelectronics - Volume 62, 15 December 2014, Pages 196-200
نویسندگان
Kun Chen, Long Wu, Xiaochun Jiang, Zhicheng Lu, Heyou Han,