کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233448 | 1470970 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A novel one-step signal amplified lateral flow strip (SA-LFS) is described for direct ultrasensitive and on-site detection of Hg2+ based on the specific recognition system of thymine-Hg2+-thymine using gold nanoparticles (AuNPs) as labeling tags. The signal was also amplified by hybridization induced dual labeling of AuNPs, which increased signal intensity of test line on strips. The presence of both probes on the LFS enabled both sensing and signal amplification to be achieved in a single step. The resulting SA-LFS is capable of rapid and ultrasensitive detection of Hg2+ with LODs of 0.005Â ppb and 0.0015Â ppb by visual observation and quantitative analysis, respectively. At least 40-fold improvement in sensitivity of the traditional LFS was achieved through this signal amplification process. The successful application of the method to the determination of Hg2+ in water sample is reported. The proposed signal amplification process may also be applicable to other LFS-based methods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 61, 15 November 2014, Pages 14-20
Journal: Biosensors and Bioelectronics - Volume 61, 15 November 2014, Pages 14-20
نویسندگان
Mengya Zhu, Ying Wang, Yi Deng, Li Yao, Samuel B. Adeloju, Daodong Pan, Feng Xue, Yucheng Wu, Lei Zheng, Wei Chen,